Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Article Title: Dipeptidyl Peptidase‐4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress
doi: 10.1161/JAHA.117.006394
Figure Lengend Snippet: DPP 4 inhibition suppressed the activated HSC proliferation in the BM . A, Gating for the LSK cell population isolated from BM cells of stress‐alone (S) and stressed‐anagliptin treatment (S‐Ana) group mice (upper panels) and subanalyzed lin − c‐Kit high Sca‐1 high CD 48 low CD 150 high HSC (lower panels). B, BM LSK and HSC numbers next to gates represent population frequencies (%) of 2 groups after 4 weeks of stress (n=6–8, Student t test). C, Ly6C high monocytes and neutrophils in the BM (n=6–8, Student t test). D, Gating for the enclosed 2 populations isolated from PB cells of 2 groups (upper panels) and identifying for neutrophils as LyG6 high CD 11b high ( top‐right quadrants ) and subanalyzing LyG6 low Ly6C high ( bottom‐right quadrants ) for monocytes as LyG6 low CD 11b high F4/80 low LyC high (lower panels). E, PB monocytes and neutrophil numbers in 2 groups of mice after 4 weeks of stress (n=6–8, Student t test). F, Representative histogram of DNA content during the cell cycle (left) and the distribution of S/M/G2 cells expressed as a percentage of total BM cells (right) (n=5, Mann–Whitney U test). G, FACS analysis show the numbers of Ki67/ + CD 150 + cells in the BM . Data are mean± SEM . * P <0.01 vs stressed‐alone control. BM indicates bone marrow; DPP4, dipeptidyl peptidase‐4; FACS, fluorescence activated cell sorter; HSC, hematopoietic stem cells; LSK, lin − sca‐1 + c‐Kit + ; PB, peripheral blood; S‐Ana, stressed‐exenatide treatment.
Article Snippet: We measured the DPP4 activity by using the DPP4 Glo Protease Assay (Promega, Madison, WI) with an aminoluciferin substrate.
Techniques: Inhibition, Isolation, MANN-WHITNEY, Control, Fluorescence
